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1968). Biotechnol. Bioeng. 10, 45. Production, Isolation, and Economics of Extracellular Enzymes Knud Aunstrup Novo Research Institute Bagsvaerd, Denmark I. Introduction II. History III. The Enzyme Industry A. Extracellular Microbial Enzymes for Industrial Use and the Producing Companies B. Market Situation C. Enzyme Costs IV. General Methods for Industrial Production of Extracellular Enzymes A. Production Strain B. Fermentation C. Recovery and Finishing V. Standardization and Control A. Standardization B.

269-326. Academic Press, New York. Clarke, P. , Houldsworth, Μ. , and Lilly, M. D. (1968). J. Gen. Microbiol 51, 225. Clarke, P. , and Lilly, M. D. (1969). Symp. Soc. Gen. Microbiol. 19, 113. Cole, J . A. (1976). In "Advances in Microbial Physiology" (A. H. Rose and D. W. ), Vol. 14, pp. 1-92. Academic Press, New York. Dean, A. C. R. (1972). J. Appl Chem. Biotechnol 22, 245. Delente, J . , Johnson, J . , Kuo, M. J . , O'Connor, R. J . , and Weeks, L. E . (1974). Biotechnol. Bioeng. 16, 1227. Demain, A.

2 Ν HC1 (containing traces of Zn, Fe, and Cu) 60 (Cayle, 1971). Lipase: 10. Wheat bran 3, soybean meal 1, water 3 (Smythe and Drake, 1949). 40 Knud Aunstrup T h e medium composition should b e balanced so that the ρ Η does not exceed the accepted limits during fermentation. This can b e achieved by the addition o f a buffer system such as phosphates or C a C 0 to the medium. Another method is to add substances whose metabolism gives rise to a change in pH, such as salts o f organic acids and nitrates that will raise the pH, or ammonium salts that will tend to lower the pH.

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