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By Joseph W. Harding

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Cardiac Receptors A radioligand receptor binding study with angiotensin in heart was first reported by Moore in 1980. He studied the binding of [ 3 H]- and [125I]Ang-II in isolated cardiocytes dispersed from 19-day-old chick embryos and reported good agreement between the K D (6 nM) for a high- 2. Actions of Angiotensin on Elements of the Vascular Wall 49 affinity binding site and the ED (5 nM) for positive inotropic activity of angiotensin in perfused hearts of 19-day-old chicks. The high-affinity binding site was not apparent in 15-day or younger chick embryos.

Chap. 19 in The Hunger for Salt, 310-337. P. Buckley. 1970. Identification of a central site of action of angiotensin II. / . Pharmacol. Exp. Ther. 175: 139-146. 26 Roberte. Speth et al. A. Rytand. 1934. Absence of vasoconstrictor substance in blood of rats with renal hypertension. Proc. Soc. Exp. Biol. Med. 32: 374- 375. , F. Rodriguez, J. Arsaut, D. Verrier and J-D. Vincent. 1982. Angiotensin II stimulates prolactin release in the rhesus monkey. Neuroendocrinol. 35: 242-247. Elie, R. -C. Panisset.

Which is a substrate for Bordetella pertussis toxin. Pretreatment with toxin should, therefore, impair angiotensin binding to its receptor. , 1985) and pretreatment with B. , 1985). , 1986) have shown that ADP-ribosylation of all the G. with B. pertussis completely blocks the inhibitory actions of angiotensin on adenylate cyclase. However, GTP still exerts a striking effect on angiotensin binding in toxin-treated liver membranes. These findings indicate that the angiotensin receptor in liver is coupled by two or more G n 2.

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